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| Registros recuperados : 5 | |
| Registros recuperados : 5 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
23/05/2022 |
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Data da última atualização: |
23/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SAN BLAS, G.; BAUDINO, E. M.; DIAS, F. M. S.; DOLIBAINA, D. R.; SPECHT, A.; CASAGRANDE, M. M.; CORNEJO, P.; GIRAUDO, W. G.; MIELKE, O. H. H. |
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Afiliação: |
GERMAN SAN BLAS; ESTELA MARIS BAUDINO; FERNANDO MAIA SILVA DIAS; DIEGO RODRIGO DOLIBAINA; ALEXANDRE SPECHT, CPAC; MIRNA MARTINS CASAGRANDE; PAULA CORNEJO; WALTER GUILLOT GIRAUDO; OLAF HERMANN HENDRIK MIELKE. |
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Título: |
Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Entomologia, v. 65, n. 4, e20210104, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ?barcode? region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ?barcodes? to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ?barcodes?, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ?barcodes? region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups. MenosAccurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ?barcode? region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ?barcodes? to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ?barcodes?, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ?barcodes? region alone can be useful for lower level phylogeny in this group, recovering a... Mostrar Tudo |
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Palavras-Chave: |
Códigos de barra de DNA; Identificação de espécies; Identificação morfológica. |
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Thesagro: |
Agrotis spp; DNA; Espécie; Identificação. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1143362/1/Alexandre-Molecular-characterization-and-phylogenetic.pdf
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Marc: |
LEADER 02670naa a2200301 a 4500
001 2143362
005 2022-05-23
008 2021 bl uuuu u00u1 u #d
100 1 $aSAN BLAS, G.
245 $aMolecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera$bNoctuidae) of South America.$h[electronic resource]
260 $c2021
520 $aAccurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ?barcode? region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ?barcodes? to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ?barcodes?, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ?barcodes? region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups.
650 $aAgrotis spp
650 $aDNA
650 $aEspécie
650 $aIdentificação
653 $aCódigos de barra de DNA
653 $aIdentificação de espécies
653 $aIdentificação morfológica
700 1 $aBAUDINO, E. M.
700 1 $aDIAS, F. M. S.
700 1 $aDOLIBAINA, D. R.
700 1 $aSPECHT, A.
700 1 $aCASAGRANDE, M. M.
700 1 $aCORNEJO, P.
700 1 $aGIRAUDO, W. G.
700 1 $aMIELKE, O. H. H.
773 $tRevista Brasileira de Entomologia$gv. 65, n. 4, e20210104, 2021.
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